Theerlenmeyer girgiza flaskwani akwati ne na musamman na al'ada don al'adun tantanin dakatarwa, kuma ana iya amfani dashi don shirya, haɗawa da adana kafofin watsa labarai daban-daban.Ana amfani da wannan al'adar da ake amfani da ita lokacin da ake haɓaka ƙwayoyin Jurkat.

Layin tantanin halitta na Jurkat an samo shi ne daga gefen jinin wani yaro ɗan shekara 14 kuma kwayar tantanin halitta ce.Layukan tantanin halitta da aka samo daga Jurkat waɗanda basu da wasu kwayoyin halitta sun riga sun kasance a bankunan al'adun tantanin halitta.Layukan lymphocyte na mutum marar mutuwa wanda aka fi amfani da shi don nazarin cutar sankarar ƙwayar cuta ta T cell, siginar T cell, da nau'ikan masu karɓar chemokine daban-daban masu saurin kamuwa da kamuwa da cuta, musamman maganganun HIV.Yana da aikace-aikace da yawa a cikin binciken ilimin halitta, kamar aikace-aikacen ƙwayoyin Jurkat don nazarin M1-RNA na ribonuclease P, da kuma nazarin M1-RNA na anti-MHC class II transcriptional activator (CIITA) don hanawa. Maganar kwayoyin MHC aji II akan farfajiyar tantanin halitta.

Lokacin al'ada Jurkat Kwayoyin a cikin erlenmeyer girgiza flasks, RPMI1640 matsakaici, 10% FBS ake bukata;ana sarrafa yawan zafin jiki a 37 ° C, 5% carbon dioxide, ƙimar PH 7.2-7.4, al'adun zafin jiki na aseptic akai-akai.Shafa da kashe barasa 75% kafin a canza shi zuwa benci mai tsabta mai tsabta, cire sel cryovial daga tankin nitrogen na ruwa, nan da nan sanya shi a cikin wanka na ruwa 37 ° C kuma girgiza tantanin halitta cryotube da sauri don narke shi da sauri.Sa'an nan, bayan centrifugation, pipetting da hadawa, da dai sauransu, an sanya shi a cikin incubator tantanin halitta don noma.

Kwayoyin suna kula da muhalli musamman.Lokacin da ake al'adar ƙwayoyin Jurkat a cikin filayen erlenmeyer cell shake, ya kamata a yi tsaftar mutum da kyau, a yi amfani da reagents mara kyau, kuma a bi ƙa'idodin aikin aseptic don guje wa gabatar da ƙwayoyin cuta da kuma shafar haɓakar tantanin halitta.