A cikin al'adun sel,kwalban al'adar salulaana amfani dashi sau da yawa a cikin akwati, yana amfani da ƙirar wuyansa mai faɗi, mai sauƙin aiki.Ci gaban sel yana buƙatar takamaiman yanayi, wanda yanayin da bakararre ke da mahimmanci.

Rashin guba da haihuwa sune sharuɗɗan farko na ƙwayoyin halitta a cikin vitro.A cikin vivo, tsarin detoxification da tsarin rigakafi na iya tsayayya da mamayewa na ƙwayoyin cuta ko wasu abubuwa masu cutarwa, amma a cikin tsarin al'adun tantanin halitta a cikin vitro, irin su kwalban al'adar kwayar halitta, rashin kariyar tsarin garkuwar jiki da asarar ikon kariya na microbial da detoxification. iya abubuwa masu cutarwa.Don tabbatar da cewa sel na iya girma da haifuwa a cikin muhalli na waje, ya zama dole a tabbatar da wurin aiki mara kyau, kyakkyawan tsaftar mutum, reagents mara kyau da kafofin watsa labarai, da kulawa mara kyau.

Tabbas, yanayin aseptic shine ɗayan mahimman yanayi don al'adun tantanin halitta a cikinkwalban al'adar salula.Sauran sun haɗa da madaidaicin zafin jiki, matsa lamba osmotic, da PH na yanayin gas, waɗanda sune ainihin yanayin da ake buƙata don haɓakar tantanin halitta.

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