• lab-217043_1280

Nawa ne ake ƙara ruwa a cikin tantanin halitta

A al'adar suspension cell,cell girgiza flaskwani nau'i ne na al'adun tantanin halitta.Ci gaban sel da aka dakatar bai dogara da saman kayan tallafi ba kuma sun girma cikin yanayin dakatarwa a cikin matsakaicin al'adu.Ta yaya za mu ƙayyade adadin ruwan da za a ƙara a cikin al'ada na gaske?

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Common bayani dalla-dalla nacell girgizasun hada da 125ml, 250ml, 500ml da 1000ml don saduwa da buƙatun nau'ikan al'adun tantanin halitta daban-daban.Misali, kwalabe 125ml da 250ml masu karamin karfi ana amfani da su ne don kananan gwaje-gwaje, yayin da 500ml da 1000ml bayani dalla-dalla ana amfani da su don gwaje-gwajen al'adun cell matsakaici.Lokacin amfani da irin wannan nau'in abubuwan amfani, ya kamata a yi amfani da girgizar girgizar don rage yawan haɓakar ƙwayar sel da kuma kula da yanayin girma mai kyau na sel.Ana buƙatar aiwatar da al'adun ƙwayoyin cuta a cikin yanayi mara kyau.Saboda haka, daflask al'adun tantanin halittaza a ba da haifuwa ta musamman kafin a yi amfani da shi don cimma tasirin babu DNA, babu RNA enzyme, da kuma abubuwan da aka samu daga dabba, samar da kyakkyawan yanayi don haɓakar tantanin halitta.

Dangane da ƙayyadaddun ƙayyadaddun kwalabe, shawarar da aka ba da shawarar cika ƙayyadaddun ƙayyadaddun kwalban daga ƙasa zuwa babba shine 30ml, 60ml, 125ml, 500ml.Gabaɗaya, ana sarrafa ƙarar maganin a cikin al'adun sel a kusan 20% -30% na jimlar ƙarar kwalban girgiza, kuma akwai layin ma'auni bayyananne akan jikin kwalban don sauƙaƙe kallon gani na iyawar maganin. .

Abin da ke sama shine shawarar adadin ruwa da aka ƙara zuwa ƙayyadaddun bayanai daban-daban nacell girgiza, wanda ba a gyara ba.Ya kamata a yi la'akari da ƙarfin gabaɗaya bisa ga halaye na haɓakar ƙwayoyin sel da yawan ƙwayar inoculation, don guje wa tasirin haɓakar tantanin halitta saboda yawan adadin ruwa da aka ƙara.

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Lokacin aikawa: Dec-14-2023