Lokacin da muke amfani da wasu abubuwan amfani da al'adun tantanin halitta, koyaushe muna fuskantar matsalar wucewar tantanin halitta.A yau, a taƙaice za mu raba tare da ku yadda ake amfani da filaye masu inganci don wucewar tantanin halitta.Lokacin da muke amfani babban inganci girgiza flasks(https://www.luoron.com/3l5l-high-efficiency-erlenmeyer-flask-product/) don wucewar tantanin halitta, akwai hanyoyi guda biyu don zaɓar daga, kamar tattara sel ta hanyar centrifugation sannan nassi, ko kai tsaye. wucewa.

Hanyar wucewa ta Centrifugal:

(1) Canja wurin sel a cikinflask mai inganci mai inganci tare da matsakaicin al'ada zuwa bututu na centrifuge don centrifugation.

(2) Yi watsi da mai girma, ƙara sabon matsakaicin al'ada zuwa ga centrifuge tube kumapipettedon samar da dakatarwar tantanin halitta.

(3) ƙidaya da allura a cikin sabbin flasks na al'ada bi da bi.

Idan an karɓi hanyar kai tsaye, bari sel da aka dakatar da su sannu a hankali su zauna a ƙasan babban gilashin girgiza mai inganci, a tsotse 1/2 ~ 2/3 na supernatant, sannan pipette don samar da dakatarwar tantanin halitta kafin wucewa.

Abin da ya kamata mu mai da hankali a yayin aikin shi ne cewa trypsin ya kamata a yi dumi, kuma zafin jiki yana kusan 37 ° C.Ya kamata gudun centrifugation ya dace.Idan saurin ya yi ƙasa da ƙasa, ba za a iya raba sel yadda ya kamata ba.Idan gudun centrifugation ya yi yawa kuma lokacin ya yi tsayi, za a matse sel, haifar da lalacewa ko ma mutuwa.Ya kamata a lura da kwayoyin halitta akai-akai, kuma idan an sami gurɓata, ya kamata a magance shi cikin lokaci.