Masana'antar tantanin halitta abu ne da ake amfani da shi a cikin al'adun sel masu girma, wanda galibi ana amfani dashi don bin al'adun tantanin halitta.Ci gaban salula yana buƙatar kowane nau'in sinadirai, to menene su?
1. Al'adu matsakaici
Matsakaicin al'adun tantanin halitta yana ba da sel a cikin masana'antar tantanin halitta tare da abubuwan gina jiki da ake buƙata don haɓaka, gami da carbohydrates, amino acid, salts inorganic, bitamin, da sauransu. , Mikiya, MEM, RPMll640, DMEM, da dai sauransu.

2. Sauran sinadaran da aka kara
Baya ga sinadarai masu gina jiki da kafofin watsa labaru daban-daban ke bayarwa, sauran abubuwan da suka dace, kamar su jini da dalilai, ana buƙatar ƙara su bisa ga sel daban-daban da manufofin al'adu daban-daban.
Serum yana ba da mahimman abubuwa kamar matrix extracellular, abubuwan haɓakawa da transferrin, kuma ana amfani da maganin ɗan tayi na yau da kullun.Matsakaicin adadin maganin da za a ƙara ya dogara da tantanin halitta da manufar binciken.10% ~ 20% serum na iya kula da saurin girma da yaduwa na sel, wanda aka sani da matsakaicin girma;Domin kiyaye jinkirin girma ko rashin mutuwa na sel, ana iya ƙara 2% ~ 5% serum, wanda ake kira al'adun kulawa.
Glutamine shine tushen nitrogen mai mahimmanci don haɓakar tantanin halitta kuma yana taka muhimmiyar rawa a cikin aiwatar da haɓakar sel da metabolism.Duk da haka, saboda glutamine yana da matukar rashin kwanciyar hankali kuma yana da sauƙin ragewa a cikin maganin, zai iya rushe kusan 50% bayan kwanaki 7 a 4 ℃, don haka glutamine yana buƙatar ƙarawa kafin amfani.
Gabaɗaya, ana amfani da kafofin watsa labaru daban-daban a al'adar tantanin halitta, amma don hana gurɓacewar ƙwayoyin cuta yayin al'ada, ana ƙara wasu adadin ƙwayoyin cuta, kamar su penicillin, streptomycin, gentamicin, da sauransu a cikin kafofin watsa labarai.