Akwai gutsuttsuran tantanin halitta da yawa bayan injin lysis na dakatarwar tantanin halitta.Yadda za a cire wadannan gutsuttsura?Bari mu dubi hanyoyi daban-daban:

1. Yi amfani da hanyar dilution.Lokacin da aka narkar da kwayoyin halitta, za su iya yaduwa, za su yi yawa, kuma tarkacen tantanin halitta zai zama ƙasa da ƙasa.
2. Akwai kuma zama na halitta.Sel sun daidaita da sauri fiye da yawancin gutsuttsura: Matsar da tantanin halitta zuwa cikincentrifuge tube, kuma lokacin da yawancin sel suka nutse, ana iya tsotse maganin na sama, sannan a saka shi cikin maganin al'ada don dakatar da sel.Ana iya amfani da wannan hanya akai-akai, yayin da kowane lokaci za ku iya lura ko ta cika bukatun ku a ƙarƙashin ma'aunin gani.
3. centrifuge low-gudunzai iya cire tarkace, gabaɗaya 700g,5mins
4. Lokacin centrifuging, a ƙarƙashin yanayin cewa adadin ƙwayoyin ya wadatar, rage lokacin centrifugation, kamar 5min, 1000rpm, maimakon 3min, 1000rpm, sannan a cire abin da ke sama, saboda necrosis da tarkace gabaɗaya suna cikin sama!Wannan aikin yana buƙatar yin sau ɗaya kawai yayin aikin wankewa kafin shiryawa!

Ko da yake akwai hanyoyi da yawa don cire tarkacen tantanin halitta, za ku ga cewa centrifugation yana ɗaya daga cikin hanyoyin da suka fi dacewa!

Kuma saboda kyallen jikin tsire-tsire da sel suna da bangon tantanin halitta wanda ya ƙunshi cellulose, hemicellulose da pectin, gabaɗaya ya zama dole a niƙa yashi quartz ko foda gilashi tare da maganin cirewar da ya dace ko bi da cellulase don cimma manufar.Rarrabuwar kwayoyin cuta ya fi wahala, saboda gaba dayan kwarangwal din bangon kwayar cutar kwayar cutar haƙiƙance haɗin gwiwa ne na peptidoglycan cystic macromolecules, mai tauri.Hanyoyin da ake amfani da su na karya bangon ƙwayoyin ƙwayoyin cuta sun haɗa da ultrasonic crushing, yashi nika, matsanancin extrusion ko maganin lysozyme.Bayan nama da sel sun karye, an zaɓi madaidaicin buffer don cire furotin da ake so.Ana cire kayan da ba a iya narkewa kamar guntun selcentrifugeko tacewa.

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