Nau'in ruwa nau'i ne da aka haɓaka tare da matsakaicin al'adun ruwa.Yana da abũbuwan amfãni na gajeren lokaci samar sake zagayowar, m kwayoyin shekaru, m inoculation, dace da masana'anta samar, da sauransu.An yi maraba da yawancin manoma.Shaker cell (https://www.luoron.com/plastic-erlenmeyer-flask-with-vent-cap-2-product/） wani nau'i ne na kayan da ake buƙata a cikin al'adar nau'in ruwa.Wajibi ne a kula da wadannan abubuwa a cikin al'ada:

1.Treparation na al'ada matsakaici bisa ga dabara na daban-daban aka gyara, a cikin akwati, narke da ruwa, zuwa kashi triangular kwalabe, kullum 250 ~ 300mL cell shake kwalban 50mL al'adu matsakaici,500ml kwalban triangle  (https://www.luoron.com/plastic-erlenmeyer-flask-with-vent-cap-2-product/）100ml al'ada matsakaici, toshe auduga toshe, tare da jarida nannade;Hakanan za'a iya yin shi da yadudduka 8 na gauze 8cm × 8cm mai rufe kwalban kwalban, a waje tare da rufe takarda mai launin ruwan kasa.
2. Autoclave sterilization Hanyar ne kullum soma ga haifuwa, wanda bukatar 121 ℃ da 30min.Cire shi kuma kwantar da shi zuwa kimanin 30 ℃.Saka shi a cikin ɗaki marar tsabta ko akwatin allura don amfani.
3. Dangane da bukatun aikin aseptic, kowane kwalban za a iya haɗa shi tare da nau'in 2 ~ 3cm2 slant, kuma kowane nau'i na slant ana iya haɗa shi da kwalabe 4 ~ 5.Zai fi kyau a kawo wasu matsakaici tare da ƙarin nau'i, don su iya iyo a saman matsakaici.Bayan allurar, rufe bakin kwalbar tare da gauze da aka toshe a asali, kuma a ɗaure shi da ƙarfi tare da zaren.
4. The inoculated iri kwalban za a iya sanya a cikin wani girgiza gado ga al'adu, ko za a iya sanya a cikin wani m zazzabi na 24 ~ 26 ℃ na 48h, sa'an nan oscillated al'adu za a iya za'ayi bayan da aerogenic mycelium aka mika a cikin al'adu matsakaici. .Mitar girgiza mai jujjuyawa shine 80 ~ 120r/min, kuma na rotary shaker shine 150 ~ 220r/min.3 ~ 4d za a iya al'ada a cikin shaker.A ƙarshen al'ada, saboda nau'i-nau'i daban-daban, matsakaicin al'ada ya bayyana launuka daban-daban, irin su naman kaza na kawa, al'adun naman kaza na enoki ya kasance rawaya mai haske;Naman kaza, matsakaicin al'adun ericius erinaceus ya kasance launin ruwan ja, da kamshin naman kaza;Matsakaicin al'adun naman gwari yana launin ruwan kasa, mai kauri da zaki.Ana iya amfani da matsakaicin al'ada don samarwa ko ƙarin al'adun faɗaɗa kawai lokacin da bushewar nauyin mycelium ya kai 10g / L kuma diamita na pellets mycelium shine 1 ~ 2mm.
 
5. Sakandare ruwa iri samar na biyu ruwa iri al'adu matsakaici samar da wannan matakin na ruwa iri, al'ada ganga ya kamata ya fi girma, da damar ba fiye da 3500mL.Bayan haifuwa da sanyaya, an saka nau'ikan ruwa na farko da aka haɗe a ciki5L shaker(https://www.luoron.com/3l5l-high-efficiency-erlenmeyer-flask-product/）a wani rabo na 5% -10% da kuma al'ada a kan shaker a jinkirin gudun.Bayan wani lokaci na al'adun oscillating, ana iya samun nau'ikan ruwa tare da rarraba iri ɗaya na microspheres da ruwa mai tsabta.
A taƙaice, lokacin amfani da shaker cell zuwa al'ada nau'in ruwa, yi aiki bisa ga hanyoyin da ke sama, kuma kula da sarrafa zafin jiki, girgiza mita da sauran abubuwa a cikin tsari don tabbatar da tasirin al'ada.
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