Filastik Erlenmeyer Shake Flask tare da hular iska

Sel suna girma a hankali a cikin Erlenmeyer Flask da Magani

Abin da ke haifar da jinkirin haɓakar ƙwayoyin sel a cikin flasks na shaker cell
Kwayoyin suna da matukar damuwa ga yanayin girma.Lokacin da muke al'adar sel, wani lokaci mukan gamu da jinkirin girmar tantanin halitta.Menene dalili?Akwai dalilai da yawa na jinkirin haɓakar ƙwayoyin sel a cikin flask ɗin tantanin halitta, galibi saboda dalilai masu zuwa:
1. Kwayoyin suna buƙatar sake daidaita su saboda canjin yanayin al'adu daban-daban ko magani.
2. The reagents ana adana ba daidai ba, kuma wasu abubuwan da ake buƙata don haɓakar tantanin halitta kamar glutamine ko abubuwan haɓakawa a cikin matsakaicin al'ada sun ƙare ko rasa ko sun lalace.
3. Akwai ƙananan ƙwayar cuta ko fungal a cikin al'ada a cikin mai girgiza tantanin halitta.
4. Matsakaicin farko na ƙwayoyin da aka yi wa allurar ya yi ƙasa da ƙasa.
5. Kwayoyin sun tsufa.
6. Mycoplasma gurbatawa
Magani da aka ba da shawara:
1. Kwatanta abun da ke cikin sabon matsakaici da matsakaici na asali, kuma kwatanta sabon magani da tsohuwar magani don tallafawa gwaje-gwajen haɓakar ƙwayoyin cuta.Bada sel su daidaita a hankali zuwa sabon matsakaici.
2. Canja cikin matsakaicin al'ada da aka shirya, ko ƙara glutamine da abubuwan haɓaka.
3. Sanya da matsakaici mara maganin rigakafi kuma maye gurbin al'ada idan an sami gurɓataccen abu.Ya kamata a adana matsakaicin al'ada a cikin 2-8 ° C a cikin duhu.Ana adana cikakkiyar matsakaici mai ɗauke da ƙwayar magani a 2-8 ° C kuma ana amfani dashi cikin makonni 2.
4. Ƙara yawan farawa na ƙwayoyin da aka yi wa allurar.
5. Sauya da sabbin ƙwayoyin iri.
6. Ware al'ada da gano mycoplasma.Tsaftace tsayawar da incubator.Idan an gano cutar mycoplasma, maye gurbin da sabon al'ada.